{"id":663,"date":"2020-02-28T04:43:00","date_gmt":"2020-02-28T04:43:00","guid":{"rendered":"https:\/\/naturepub.org\/?p=663"},"modified":"2020-06-20T04:48:54","modified_gmt":"2020-06-20T04:48:54","slug":"protective-effects-of-vitamin-d-and-metformin-against-hematological-biochemical-and-histopathological-alterations-in-induced-diabetic-rats","status":"publish","type":"post","link":"https:\/\/naturepub.org\/index.php\/2020\/02\/28\/protective-effects-of-vitamin-d-and-metformin-against-hematological-biochemical-and-histopathological-alterations-in-induced-diabetic-rats\/","title":{"rendered":"Protective effects of Vitamin D and Metformin against Hematological, Biochemical and Histopathological Alterations in Induced Diabetic Rats"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><strong>ABSTRACT<\/strong><strong><\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Our study was designed to evaluate the association between vitamin D and diabetes mellitus in induced diabetic rats of some hematological and biochemical parameters as well as histopathological examination. The study was conducted on a total sixty male albino rats weighing (120-170 g , BW) , that were divided into two groups, the first group fed on normal diet,&nbsp; and the second group&nbsp; fed on&nbsp; high-fat diet for 2 weeks predispose cause to diabetic induction. Then intraperitoneal injection of Streptozotocin (STZ) to induce diabetes. The experimental rats randomly divided into six equals groups, as follow: 1<sup>st<\/sup> group (-ve control) non-supplemented non-diabetic, 2<sup>nd<\/sup> group &nbsp;diabetic untreated (DM) , 3<sup>rd<\/sup> group diabetic treated with vitamin D alone (DM+VD), 4<sup>th<\/sup> group&nbsp; diabetic treated with metformin (DM+ Met) , 5<sup>th <\/sup>group (DM+ VD+ Met) diabetic treated with vitamin D and metformin, 6<sup>th <\/sup>group (VD) rats supplemented with vitamin D. The treatment lasted for 4 weeks then whole blood, serum samples,&nbsp; and tissue specimens were collected for hematological, some serum biochemical analysis and histopathological examination respectively. Our findings revealed that the treatment of diabetic induced rats with both vitamin D and metformin had a significant effect on the hematological parameters except total leukocytic and lymphocytic counts, which were markedly increased. Moreover, a significant increase in the serum hepatic enzymes activities (ALT, AST, and ALP). Meanwhile, a significant reduction in TP, ALB, GLO, A\/G ratio, Total Bilirubin , Direct Bilirubin&nbsp; and&nbsp; Indirect Bilirubin .&nbsp;<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Keywords:<\/strong> Vitamin D; Diabetes Mellitus (DM); Streptozotcin (STZ); hematology, biochemical parameters and histopathological examination.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>ABSTRACT Our study was designed to evaluate the association between vitamin D and diabetes mellitus in induced diabetic rats of some hematological and biochemical parameters as well as histopathological examination. The study was conducted on a total sixty male albino rats weighing (120-170 g , BW) , that were divided into two groups, the first group fed on normal diet,&nbsp; and the second group&nbsp; fed&hellip; <\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[49,48],"tags":[76,78,80,79,77],"class_list":["post-663","post","type-post","status-publish","format-standard","hentry","category-issue-one-volume-seven","category-volume-seven","tag-biochemical-parameters-and-histopathological-examination","tag-diabetes-mellitus-dm","tag-hematology","tag-streptozotcin-stz","tag-vitamin-d","clearfix"],"_links":{"self":[{"href":"https:\/\/naturepub.org\/index.php\/wp-json\/wp\/v2\/posts\/663","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/naturepub.org\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/naturepub.org\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/naturepub.org\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/naturepub.org\/index.php\/wp-json\/wp\/v2\/comments?post=663"}],"version-history":[{"count":1,"href":"https:\/\/naturepub.org\/index.php\/wp-json\/wp\/v2\/posts\/663\/revisions"}],"predecessor-version":[{"id":665,"href":"https:\/\/naturepub.org\/index.php\/wp-json\/wp\/v2\/posts\/663\/revisions\/665"}],"wp:attachment":[{"href":"https:\/\/naturepub.org\/index.php\/wp-json\/wp\/v2\/media?parent=663"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/naturepub.org\/index.php\/wp-json\/wp\/v2\/categories?post=663"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/naturepub.org\/index.php\/wp-json\/wp\/v2\/tags?post=663"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}