{"id":666,"date":"2020-02-28T04:49:00","date_gmt":"2020-02-28T04:49:00","guid":{"rendered":"https:\/\/naturepub.org\/?p=666"},"modified":"2020-06-20T04:52:59","modified_gmt":"2020-06-20T04:52:59","slug":"assessment-of-genetic-polymorphisms-in-commercial-broiler-breeds-using-rapd-pcr-as-molecular-markers","status":"publish","type":"post","link":"https:\/\/naturepub.org\/index.php\/2020\/02\/28\/assessment-of-genetic-polymorphisms-in-commercial-broiler-breeds-using-rapd-pcr-as-molecular-markers\/","title":{"rendered":"Assessment of Genetic Polymorphisms in Commercial Broiler Breeds using RAPD-PCR as Molecular Markers"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><strong>ABSTRACT<\/strong><strong><\/strong><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">RAPD-PCR was applied to evaluate the phylogenetic relationship and genetic variation among three chicken breeds; Cobb, Avian, and Ross. A total of seven arbitrary short primers were used and they generated polymorphic, reproducible and score able bands. 42 bands were produced from these primers. Out of these bands, 3 (7.14 %) were recognized as monomorphic and 18 (42.85 %) as polymorphic bands. The highest percentage (67%) of polymorphic bands was generated by OPC-1 primer, while the lowest percentage (20%) of polymorphic bands was generated by OPA-20 primer. Interbreed of band sharing frequencies were 0.82\u00b10.13 between Avian-Cobb, 0.76\u00b10.19 between Avian-Ross and 0.85\u00b10.09 between Cobb-Ross. Three primers (OPA-8, OPA-10, and OPA-20) were specified for Ross, Avian and Cobb breeds respectively. The study recommends the use of RAPD- PCR to detect the genetic changes and relations among broiler chicken breeds.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong>Keywords:<\/strong> Poultry, RAPD- PCR, Genetic Variability, Phylogenetic.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>ABSTRACT RAPD-PCR was applied to evaluate the phylogenetic relationship and genetic variation among three chicken breeds; Cobb, Avian, and Ross. A total of seven arbitrary short primers were used and they generated polymorphic, reproducible and score able bands. 42 bands were produced from these primers. Out of these bands, 3 (7.14 %) were recognized as monomorphic and 18 (42.85 %) as polymorphic bands. The highest&hellip; <\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[49,48],"tags":[83,84,81,82],"class_list":["post-666","post","type-post","status-publish","format-standard","hentry","category-issue-one-volume-seven","category-volume-seven","tag-genetic-variability","tag-phylogenetic","tag-poultry","tag-rapd-pcr","clearfix"],"_links":{"self":[{"href":"https:\/\/naturepub.org\/index.php\/wp-json\/wp\/v2\/posts\/666","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/naturepub.org\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/naturepub.org\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/naturepub.org\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/naturepub.org\/index.php\/wp-json\/wp\/v2\/comments?post=666"}],"version-history":[{"count":1,"href":"https:\/\/naturepub.org\/index.php\/wp-json\/wp\/v2\/posts\/666\/revisions"}],"predecessor-version":[{"id":668,"href":"https:\/\/naturepub.org\/index.php\/wp-json\/wp\/v2\/posts\/666\/revisions\/668"}],"wp:attachment":[{"href":"https:\/\/naturepub.org\/index.php\/wp-json\/wp\/v2\/media?parent=666"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/naturepub.org\/index.php\/wp-json\/wp\/v2\/categories?post=666"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/naturepub.org\/index.php\/wp-json\/wp\/v2\/tags?post=666"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}