Molecular Characterization of Multidrug-Resistant Salmonella species from Meat in Delta Area, Egypt


A total of 180 samples from meat (30 fresh slaughtered poultry, 30 frozen poultry, 30 poultry organs, 30 fresh beef meat, 30 frozen beef meat and 30 beef organs) were obtained randomly from slaughterhouses, restaurants and supermarkets at Delta area, Egypt. Collected samples were examined for the presence of Salmonella spp. Biochemical and serological identification were applied, molecular characterization using multiplex PCR technique was used for confirming the serotyping of Salmonella spp. and identification of antimicrobial resistance genes. Results revealed that incidence of Salmonella spp. was 8.3% (15 isolates), of these 15 isolates, Salmonella typhimurium was the highly isolated serovar represented in (9) isolates, Salmonella paratyphi A (3), Salmonella enteritidis (2) and Salmonella kentucky (1). Antibiogram resistance phenotype showed that ciprofloxacin and chloramphenicol were most effective against strains while ampicillin and cefotaxime were the lowest effective against strains. Then, molecular characterization of β-lactamases resistance genes showed presence of blaTEM gene in 6 isolates, blaCMY in 2 isolates, blaCTX-M in 12 isolates where blaOXA and blaSHV genes could not be identified.

Keywords: β-lactamases, Egypt, meat, Salmonella.