ABSTRACT

Our study was designed to evaluate the association between vitamin D and diabetes mellitus in induced diabetic rats of some hematological and biochemical parameters as well as histopathological examination. The study was conducted on a total sixty male albino rats weighing (120-170 g , BW) , that were divided into two groups, the first group fed on normal diet,  and the second group  fed on  high-fat diet for 2 weeks predispose cause to diabetic induction. Then intraperitoneal injection of Streptozotocin (STZ) to induce diabetes. The experimental rats randomly divided into six equals groups, as follow: 1^st group (-ve control) non-supplemented non-diabetic, 2^nd group  diabetic untreated (DM) , 3^rd group diabetic treated with vitamin D alone (DM+VD), 4^th group  diabetic treated with metformin (DM+ Met) , 5^th group (DM+ VD+ Met) diabetic treated with vitamin D and metformin, 6^th group (VD) rats supplemented with vitamin D. The treatment lasted for 4 weeks then whole blood, serum samples,  and tissue specimens were collected for hematological, some serum biochemical analysis and histopathological examination respectively. Our findings revealed that the treatment of diabetic induced rats with both vitamin D and metformin had a significant effect on the hematological parameters except total leukocytic and lymphocytic counts, which were markedly increased. Moreover, a significant increase in the serum hepatic enzymes activities (ALT, AST, and ALP). Meanwhile, a significant reduction in TP, ALB, GLO, A/G ratio, Total Bilirubin , Direct Bilirubin  and  Indirect Bilirubin . 

Keywords: Vitamin D; Diabetes Mellitus (DM); Streptozotcin (STZ); hematology, biochemical parameters and histopathological examination.